The Sword Difference
Sword Bio offers the experience and expertise to support genomics-based assays using accepted and proven platforms in the industry.
We support our clients along the entire drug development cycle. Let us help develop, validate and test your samples during your research, preclinical and clinical trials.
How Our Team Can Help
- SiRNA/mRNA
- CAR-T therapy
- AAV viral services (Purity/safety testing for manufacturing/Preclinical and Clinical projects)
- Evaluations of DNA or RNA therapeutics, including biodistribution/Vector shedding and quantitation of transgene for gene and cell therapies
- NGS library quantification & verification
- VCN (Vector copy number)
- Detection of DNA or RNA aptamers in Liquid biopsies for rare allele/mutation detection and Cell/Vectors in Plasma
- Gene expression analysis
- Cytokine/Chemokine mRNA in immune response
- Biomarker assessment
Systems
qPCR or Real Time-PCR
qPCR stands for quantitative polymerase chain reaction and is a PCR technology for quantifying DNA or RNA.
qPCR measures gene expression and will tell you how much of a specific type of DNA or RNA there is using different techniques.
dPCR or digital-PCR
Digital PCR (dPCR) delivers precise and multiplexed PCR analysis for absolute quantification.
Depending on the sample state (low copy number) and need for precision, using dPCR can offer benefits compared to qPCR.
Digital PCR Technology V.S. Droplet Digital PCR
Some emulsion-based or droplet digital PCR (ddPCR) technologies lack consistency because they depend on an inherently stochastic process—fluid shearing—to create individual compartments. This can result in the total number of compartments generated per ddPCR reaction being highly variable.
Digital PCR (dPCR) technology does not rely on fluidic shearing used in droplets or a physical displacement of excess reagents to form compartments or microchambers.
So, besides delivering superior consistency, reduced reagent waste, and a simplified workflow, dPCR technology provides greater volume precision, higher numbers of microchambers generated, and more accurate quantification.