Sword Assay for Human IL-1β

Interleukin-1 is a pro-inflammatory cytokine that can be expressed in two separate isoforms: IL-1α and IL-1β.1 IL-1β is expressed by dendritic cells and acts through the TNFα signaling pathway to induce expression of several inflammatory cytokines2, 3; it has therefore been identified as both a biomarker for inflammation diagnosis and a target for inflammation therapy.

Current ELISAs lack the required sensitivity to quantify IL-1β in normal healthy donor serum for proper comparison with diseased individuals. Sword has changed that by developing a reagent based on the principle of Raman Resonance that can easily be inserted into a standard IL-1β ELISA with greatly enhanced sensitivity.

This Sword Assay has been optimized for use with the R&D Systems DuoSet ELISA for Human IL-1β (Catalog No. DY201). This protocol allows the user to use 50 µL sample sizes instead of 100 µL as specified in the DuoSet literature.

Assay Type:

Sandwich ELISA

Product #:

SB-HIL2301-05

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Sensitivity

Precision

Recovery

Quantification

Sensitivity

Precision

Recovery

Quantification

Citations

Weber A, et al: Interleukin-1beta (IL-1beta) processing pathway. Sci Signal 2010, 3:cm2.
Luft T, et al: IL-1 beta enhances CD40 ligand-mediated cytokine secretion by human dendritic cells (DC): a mechanism for T cell-independent DC activation. Journal of immunology 2002, 168:713-22.
Zhang et al: Interleukin-1beta induces macrophage inflammatory protein-1beta expression in human hepatocytes. Cell Immunol 2003, 226:45-53.

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Sword Assay for Human IL-1β

Assay Type:

Product #:

See the Sword Performance Difference

Figure 1. Sword Assay for Human IL-1β

Table 1. Precision of IL-1β Quantification in Human Serum with Sword Assay

Table 2. Precision of IL-1β Quantification in Human Plasma EDTA with Sword Assay

Table 3. Spike Recovery with Sword Assay for Human IL-1β in Serum

Table 4. Spike Recovery with Sword Assay for Human IL-1β in Plasma EDTA

Table 5. Quantification of IL-1β in Healthy Human Serum

Table 6. Quantification of IL-1β in Healthy Human Plasma

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