IL-33 is constitutively expressed in the cell nuclei of normal healthy individuals.4 Damage to the cell causes the release of IL-33 into the extracellular matrix which then signals the beginning of the inflammatory process.5 As a result, IL-33 functions as an alarmin and subsequently has been identified as a strong biomarker candidate for inflammation.
This Sword Assay has been optimized for use with the R&D Systems Quantikine ELISA for Human IL-33 (Catalog No. D3300). This protocol allows the user to use 100 μL sample sizes.
Table 1. IL-33 levels were quantifies in human serum from healthy donors using the R&D Systems Human IL-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33. Donor samples were tested in duplicate in three separate runs.
Table 2. IL-33 levels were quantified in human plasma EDTA from healthy donors using the R&D Systems Human IL-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33. Donor samples were tested in duplicate in three separate runs.
Table 5. Human IL-33 was quantifies in human serum from twelve healthy donors using the R&D Systems Human IL-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33. Measured human IL-33 levels varied from 0.15 pg.ml to 87.67 pg/ml. Mean and median human IL-33 levels were 8.63 pg/ml and 1.08 pg/ml, respectively.
Table 6. Human IL-33 was quantified in human plasma EDTA from twelve healthy donors using the R&D Systems Human IL-33 Quantikine ELISA (D3300) with Sword Assay for Human IL-33. Measured human IL-33 levels varied from 0.19 pg/ml to 9.03 pg/ml. Mean and median human IL-33 levels were 2.27 pg/ml and 1.64 pg/ml respectively.
Citations
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