In response to tumor-induced hypoxia there have been high levels of VEGF found in various types of tumors, but this is not the case in normal tissue.3 Elevated levels of VEGF result in bronchial asthma, diabetic retinopathy, rheumatoid arthritis, and tumor growth and, the biological properties of VEGF make it an important therapeutic target.
TThis Sword Assay has been optimized for and requires the R&D Systems Human VEGF DuoSet ELISA (Catalog No. DY293B).
Table 1. VEGF levels were quantified in human serum from healthy donors using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VERGF. Donor samples were tested in duplicate in three separate runs.
Table 2. VEGF levels were quantified in human plasma EDTA from healthy donors using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF. Donor samples were tested in duplicate in three separate runs.
Table 3. Human VEGF Reference Standard was spiked into pooled human serum from healthy donors. Human VEGF levels were quantified using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF.
Table 4. Human VEGF Reference Standard was spiked into pooled human plasma EDTA from healthy donors. Human VEGF levels were quantified using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF.
Table 5. Human VEGF was quantified in human serum from twelve healthy donors using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF. Measured human VEGF levels varied from not detectable (ND) to 14.33 pg/ml. Mean and median human VEGF levels were 4.59 pg/ml and 4.40 pg/ml, respectively.
Table 6. Human VEGF was quantified in human plasma EDTA from twelve healthy donors using the R&D Systems Human VEGF DuoSet ELISA (DY293B) with Sword Assay for Human VEGF. Measured human VEGF levels varied from 0.19 pg/ml to 10.02 pg/ml. Mean and median human VEGF levels were 2.79 pg/ml and 2.12 pg/ml, respectively.
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