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Sword Nuc-Cyte™ DNA-Binding Assays

Tap Our Assay Expertise to Improve ADA Analysis

Sword Bio has optimized a number of proprietary assays to support the analysis of anti-drug antibodies (ADAs) with significantly improved sensitivity, precision, and performance. If you would like to obtain an assay as a customized solution for your ADA project, you can request more information here.

In response to our client’s needs, Sword Method Development Scientists have developed our proprietary Nuc-Cyte DNA-Binding Platform for the identification and quantitation of cellular transcription factors.

Transcription factors (TFs) are cellular proteins that regulate the transcription of DNA by binding to specific nucleic acid regulatory sequences within genes.

Their role in the regulation of gene expression is critical to fundamental cellular processes, such as cell differentiation, development, growth, replication, survival, and death. 

Increasingly, pharmaceutical  and diagnostic companies are investigating TFs as potential targets for novel small molecule therapeutics or as diagnostic biomarkers of disease prognosis or status, based on their key role in the activation or repression of genes associated with a specific pathological or disease state.

To promote the accurate and precise detection and quantitation of TFs, we developed the Nuc-Cyte™ Platform for DNA Binding Assays (the Nuc-Cyte™ Platform).   The Nuc-Cyte™ Platform consists of:

  • A synthetic, biotinylated oligonucleotide (oligo) whose nucleotide sequence corresponds to the binding sequence for the specific TF of interest.
  • A primary antibody (specific for the TF-oligo complex)
  • A secondary antibody conjugated to horseradish peroxidase (HRP)
  • Our proprietary Sword Swift™ Detection Reagents that generate a resonance Raman based assay signal with excellent to signal-to-noise ratio and stability (for up to 120 minutes at room temperature).

Nuc-Cyte™ DNA-binding immunoassays are:

  • Versatile: the assay format can be readily adapted to quantitate different TFs by utilizing different oligos specific for different TFs
  • Rapid: can be completed within 4 to 6 hours
  • High throughput: multiple samples can be analyzed simultaneously in a microtiter plate-based format
  • Highly sensitive, accurate, and precise: due to the use of our Sword Swift™ Detection Reagents, Nuc-Cyte™ DNA-binding immunoassays highly are sensitive, accurate, and precise in comparison to colorimetric-based immunoassays.

Contact us today to discuss how our proprietary Sword Swift™ Method Development Process, Sword Swift™ Detection Reagents, and our scientific method development expertise can be utilized to speed your drug or diagnostic assay development processes.

What is Sword SWIFT?

Our program enables you to get more out of your existing assays to answer critical development questions faster.


Learn more about Sword’s patented technology and how it can be optimally configured for your specific matrix needs.